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PhD Student: Jose Alfonso Marrugo

Directors: ICREA Prof. Arben Merkoçi (Group Leader), Dr Andrew Piper (Postdoctoral Researcher) and Dr Giulio Rosati (Senior Postdoctoral Researcher) in the Nanobioelectronics and Biosensors Group at ICN2.

Short Abstract: This thesis explores the development of innovative biosensing systems using aptamers and molecularly imprinted polymers (MIPs) to accurately detect small molecules. The main focus is on their application in point-of-care diagnostics. The research addresses significant challenges in traditional biorecognition methods, particularly in detecting small-molecule biomarkers associated with Acute-on-Chronic Liver Failure (ACLF). The study emphasizes the synthesis, characterization, and electrochemical detection of MIPs and MIP nanoparticles, as well as the selection of aptamers through advanced SELEX techniques, including magnetic bead-based partitioning and next-generation sequencing. By integrating these advanced biorecognition elements, the thesis aims to contribute to diagnostic and monitoring capabilities in clinical and environmental settings, laying the groundwork for future innovations in biosensing technologies.